This work comprises two parts. In the first part, three chemometric assisted spectrophotometric techniques namely;\r\nclassical least-squares (CLS), principal component regression (PCR) and partial least-squares (PLS) were applied for the\r\ndetermination of binary mixtures containing candesartan cilexetil (CND) and hydrochlorothiazide (HCZ) in methanol. In the\r\nsecond part, a precise and stability-indicating RP-HPLC was developed for the determination of CND and HCZ in ternary mixture\r\nwith candesartan cilexetil degradation product (CDP) using indapamide (IND) as an internal standard. The chromatographic\r\nseparation was carried out on a C18 column (250 mm x 4.6 mm, 5?m) using a mobile phase consisting of acetonitrile: potassium\r\ndihydrogen phosphate (40 mM, adjusted to pH 8.5 with potassium hydroxide) (48:52 v/v) with 0.1% triethylamine in each\r\ncomponent as ion pairing agent. Flow rate was maintained at 0.9 ml min-1 while UV detection was carried out at 260 nm.\r\nLinearity, accuracy and precision were found to be acceptable over the concentration ranges of 3.2ââ?¬â??13.2 ?g ml?1 and 2.5ââ?¬â??10 ?g\r\nml?1 for CND and HCZ, respectively in case of chemometric methods as well as 6.4 ââ?¬â?? 41.6 ?g ml-1 and 5 ââ?¬â?? 32.5 ?g ml-1 for CND and\r\nHCZ, respectively in case of the LC method. The Validated methods proved to be specific, robust and accurate for the quality\r\ncontrol of the cited drugs in pharmaceutical preparations.
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